Comparison of the three optical platforms for measurement of cellular respiration.
Identifieur interne : 000107 ( Main/Exploration ); précédent : 000106; suivant : 000108Comparison of the three optical platforms for measurement of cellular respiration.
Auteurs : Alina V. Kondrashina [Irlande (pays)] ; Vladimir I. Ogurtsov [Irlande (pays)] ; Dmitri B. Papkovsky [Irlande (pays)]Source :
- Analytical biochemistry [ 1096-0309 ] ; 2014.
Abstract
We compared three optical platforms for measurement of cellular respiration: absolute oxygen consumption rates (OCRs) in hermetically sealed microcuvettes, relative OCRs measured in a 96-well plate with oil seal, and steady-state oxygenation of cells in an open 96-well plate. Using mouse embryonic fibroblasts cell line, the phosphorescent intracellular O2 probe MitoXpress-Intra, and time-resolved fluorescence reader, we determined algorithms for conversion of relative OCRs and cell oxygenation into absolute OCRs, thereby allowing simple high-throughput measurement of absolute OCR values.
DOI: 10.1016/j.ab.2014.09.001
PubMed: 25233002
Affiliations:
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Ogurtsov</name><affiliation wicri:level="1"><nlm:affiliation>Tyndall National Institute, Dyke Parade, Cork, Ireland.</nlm:affiliation><country xml:lang="fr">Irlande (pays)</country><wicri:regionArea>Tyndall National Institute, Dyke Parade, Cork</wicri:regionArea><wicri:noRegion>Cork</wicri:noRegion></affiliation></author><author><name sortKey="Papkovsky, Dmitri B" sort="Papkovsky, Dmitri B" uniqKey="Papkovsky D" first="Dmitri B" last="Papkovsky">Dmitri B. Papkovsky</name><affiliation wicri:level="1"><nlm:affiliation>School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland. Electronic address: d.papkovsky@ucc.ie.</nlm:affiliation><country xml:lang="fr">Irlande (pays)</country><wicri:regionArea>School of Biochemistry and Cell Biology, University College Cork, Cork</wicri:regionArea><wicri:noRegion>Cork</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:25233002</idno><idno type="pmid">25233002</idno><idno type="doi">10.1016/j.ab.2014.09.001</idno><idno type="wicri:Area/PubMed/Corpus">000012</idno><idno type="wicri:Area/PubMed/Curation">000012</idno><idno type="wicri:Area/PubMed/Checkpoint">000012</idno><idno type="wicri:Area/Ncbi/Merge">000209</idno><idno type="wicri:Area/Ncbi/Curation">000209</idno><idno type="wicri:Area/Ncbi/Checkpoint">000209</idno><idno type="wicri:Area/Main/Merge">000108</idno><idno type="wicri:Area/Main/Curation">000107</idno><idno type="wicri:Area/Main/Exploration">000107</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Comparison of the three optical platforms for measurement of cellular respiration.</title><author><name sortKey="Kondrashina, Alina V" sort="Kondrashina, Alina V" uniqKey="Kondrashina A" first="Alina V" last="Kondrashina">Alina V. Kondrashina</name><affiliation wicri:level="1"><nlm:affiliation>School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland.</nlm:affiliation><country xml:lang="fr">Irlande (pays)</country><wicri:regionArea>School of Biochemistry and Cell Biology, University College Cork, Cork</wicri:regionArea><wicri:noRegion>Cork</wicri:noRegion></affiliation></author><author><name sortKey="Ogurtsov, Vladimir I" sort="Ogurtsov, Vladimir I" uniqKey="Ogurtsov V" first="Vladimir I" last="Ogurtsov">Vladimir I. Ogurtsov</name><affiliation wicri:level="1"><nlm:affiliation>Tyndall National Institute, Dyke Parade, Cork, Ireland.</nlm:affiliation><country xml:lang="fr">Irlande (pays)</country><wicri:regionArea>Tyndall National Institute, Dyke Parade, Cork</wicri:regionArea><wicri:noRegion>Cork</wicri:noRegion></affiliation></author><author><name sortKey="Papkovsky, Dmitri B" sort="Papkovsky, Dmitri B" uniqKey="Papkovsky D" first="Dmitri B" last="Papkovsky">Dmitri B. Papkovsky</name><affiliation wicri:level="1"><nlm:affiliation>School of Biochemistry and Cell Biology, University College Cork, Cork, Ireland. Electronic address: d.papkovsky@ucc.ie.</nlm:affiliation><country xml:lang="fr">Irlande (pays)</country><wicri:regionArea>School of Biochemistry and Cell Biology, University College Cork, Cork</wicri:regionArea><wicri:noRegion>Cork</wicri:noRegion></affiliation></author></analytic><series><title level="j">Analytical biochemistry</title><idno type="eISSN">1096-0309</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We compared three optical platforms for measurement of cellular respiration: absolute oxygen consumption rates (OCRs) in hermetically sealed microcuvettes, relative OCRs measured in a 96-well plate with oil seal, and steady-state oxygenation of cells in an open 96-well plate. Using mouse embryonic fibroblasts cell line, the phosphorescent intracellular O2 probe MitoXpress-Intra, and time-resolved fluorescence reader, we determined algorithms for conversion of relative OCRs and cell oxygenation into absolute OCRs, thereby allowing simple high-throughput measurement of absolute OCR values.</div></front></TEI><affiliations><list><country><li>Irlande (pays)</li></country></list><tree><country name="Irlande (pays)"><noRegion><name sortKey="Kondrashina, Alina V" sort="Kondrashina, Alina V" uniqKey="Kondrashina A" first="Alina V" last="Kondrashina">Alina V. Kondrashina</name></noRegion><name sortKey="Ogurtsov, Vladimir I" sort="Ogurtsov, Vladimir I" uniqKey="Ogurtsov V" first="Vladimir I" last="Ogurtsov">Vladimir I. Ogurtsov</name><name sortKey="Papkovsky, Dmitri B" sort="Papkovsky, Dmitri B" uniqKey="Papkovsky D" first="Dmitri B" last="Papkovsky">Dmitri B. Papkovsky</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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